Goats will be tested for CAEV and milk cultured for bacteria for intramammary infection (IMI) at enrollment (freshening), mid-lactation (150 days in milk) and the end of lactation. Goats with clinically enlarged joints (as determined by the investigator) will be excluded from the study. DHIA records will be reviewed on a monthly basis for change in SCC and to record 305-day milk yieldĪnd composition data. Breed, parity, kidding group (month) and prior mastitis history will be recorded. Serum ELISA test for CAEV antibody and PCR of peripheral blood mononuclear cells (PBMC) for CAEV pro-viral DNA (as previously described) will be used to determine infection status at enrollment and to categorize goats as uninfected, infected during the study period,or infected throughout the study period. We expect an 8-month enrollment period (starting at beginning of fall kidding) and 10 month follow-up per animal. Animals will be pre-screened for enrollment as they approach kidding due date. Out of office hours please contact the duty press officer on 028 9037 8110.Project Methods CAEV-infected does (n=140), as determined by positive serum CAEV-ELISA and PBMC CAEV-PCR,without signs of joint enlargement (sign of clinical CAEV infection) and CAEV-uninfected does (n=160), as determined by negative CAEV-ELISA and PBMC CAEV-PCR tests) will be will be enrolled at time of freshening.
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